Journal: Frontiers in Microbiology
Article Title: Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2
doi: 10.3389/fmicb.2023.1073789
Figure Lengend Snippet: The nuclear translocation of S protein and S mRNA includes both the outer surface and inside of the nucleus. Separate slides (see ) were imaged under a Leica Stellaris confocal microscope (Leica) using a 63x oil objective. The images were then deconvolved using Huygen Essential deconvolution software (Scientific Volume Imaging). Using the surface rendering function of an image processing IMARIS software. (A) S mRNA (red) on the nuclear surface (top) and inside the nucleus (bottom). White arrows indicate S protein on the nuclear surface (top image) or inside the nucleus (bottom image). (B) S protein (green) on the nuclear surface (top image) and inside the nucleus (bottom image). White arrows indicate S protein on the nuclear surface (top image) or inside the nucleus (bottom image). (C) The total distribution of S mRNA and S protein in the cells. The data were obtained by combining multiple images from an independent experiment. (D) The total colocalization between S mRNA and S protein in the cells. The data were obtained by combining multiple images from an independent experiment.
Article Snippet: To confirm the physical apposition between S mRNA and the nucleus by comparing their distributions in fluorescent images, we used the spot-to-spot colocalization function in Imaris image analysis software (Oxford Instruments).
Techniques: Translocation Assay, Microscopy, Software, Imaging